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Item #:
180155808
Tris Base Ultrapure, 5 kg
Item #:
180155808
SHP 493
Price Details
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Experience the highest quality with our ULTRA PURE Tris base, ensuring reliability in your scientific endeavors.
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What Stands Out
High Purity
This ultrapure Tris Base ensures minimal impurities, making it ideal for sensitive biological experiments and reliable buffer solutions in molecular biology applications.
Large Quantity
The 5 kg pack offers exceptional value, reducing the need for frequent reorders and ensuring consistent availability for laboratories with high usage rates.
Versatile Applications
Suitable for various applications in biochemistry and molecular biology, this Tris Base is ideal for buffer preparation, enhancing experimental flexibility for diverse research needs.
Product Details
| Item Weight | 11.02 lbs (5 kg) |
Who Should Buy?
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Laboratory Researchers
Ideal for researchers requiring high-purity Tris Base for biological and chemical experiments in a laboratory setting.
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Molecular Biologists
Useful for molecular biologists working on buffers, electrophoresis, or DNA/RNA experiments needing reliable pH maintenance.
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Academic Institutions
Perfect for universities and colleges offering courses in chemistry or biology that need dependable lab supplies.
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Casual Users
Not suitable for individuals without laboratory skills or knowledge, as proper use requires scientific understanding.
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Non-Scientific Use
Not recommended for applications outside of scientific research, as its purpose is strictly academic or laboratory-related.
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Small Scale Projects
Purchasing 5 kg is excessive for hobbyists or small projects that require minimal quantities of buffering agents.
Product Description
Tris Base Ultrapure, 5 kg
Have any Query? Chat with us
Customer Questions & Answers
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Question:
What is Tris Base used for?
Answer: Tris Base is commonly utilized in biochemical and molecular biology laboratories as a buffering agent. Its ability to maintain a stable pH is critical during various experiments, including DNA/RNA electrophoresis, protein assays, and enzyme reactions. By providing a controlled environment, Tris helps ensure the reliability of experimental results, supporting precise analytical outcomes. -
Question:
How should Tris Base be stored?
Answer: Tris Base should be stored in a cool, dry place away from direct sunlight to maintain its integrity. Proper storage is essential since exposure to moisture and heat can lead to degradation of the product. It’s advisable to keep it in its original container, tightly sealed, and labeled, to avoid contamination and ensure its efficacy for future experiments or applications. -
Question:
What is the difference between Tris Base and other buffers?
Answer: Tris Base is distinguished from other buffering agents due to its effective pH range (typically 7.0 to 9.0) and low absorbance in UV light, making it ideal for biochemical applications. Unlike phosphates, which can participate in reactions, Tris maintains a stable environment without interfering with many biochemical processes. This makes it highly preferred in molecular biology settings for preparing solutions. -
Question:
Can Tris Base be used in protein purification?
Answer: Yes, Tris Base is commonly used in protein purification techniques such as affinity chromatography and gel filtration. As a buffering agent, it helps to maintain pH stability, which is vital for preserving protein structure and function. Its role in controlling ionic strength also aids in optimizing separation processes during protein extraction and analysis. -
Question:
Is Tris Base suitable for cell culture applications?
Answer: Tris Base can be used in specific cell culture applications, primarily as a buffer in various growth media. However, care should be taken as its pH-stabilizing properties may affect the media's composition. It’s often used in such contexts to ensure precise pH maintenance during experimental setups where consistent cellular environments are crucial for successful cell growth and experimentation. -
Question:
What precautions should be taken when handling Tris Base?
Answer: When handling Tris Base, it is essential to wear appropriate personal protective equipment like gloves and goggles to avoid skin and eye irritation. It's also prudent to work in a well-ventilated area or fume hood. Proper lab safety protocols should be followed to prevent inhalation or accidental ingestion, ensuring a safe workspace while working with chemical reagents. -
Question:
Can Tris Base be used in preparing electrophoresis buffers?
Answer: Absolutely! Tris Base is a key component in preparing both TAE and TBE buffers for electrophoresis. These buffers are crucial in maintaining a stable pH during the separation of nucleic acids. Its low conductivity allows for efficient migration of DNA/RNA, providing clarity in band visualization after gel electrophoresis, which is essential for successful molecular biology experiments. -
Question:
What is the solubility of Tris Base in water?
Answer: Tris Base is highly soluble in water, typically dissolving readily at concentrations required for most laboratory applications. This high solubility ensures that solutions can be prepared with precision, making it an effective buffering agent in experiments. It is essential to prepare a concentrated stock solution and then dilute it as needed for specific tasks, optimizing performance in various biochemical assays. -
Question:
How does Tris Base compare to PBS?
Answer: Tris Base and Phosphate-Buffered Saline (PBS) serve different purposes in laboratory applications. While PBS is used primarily for maintaining isotonic conditions for cells, Tris Base is favored for its buffering capacity over a wider pH range. Researchers often choose Tris when more stringent pH control is necessary or in cases where phosphate interference might be problematic, such as in certain enzymatic studies. -
Question:
Where can I buy Tris Base (Ultrapure, 5 kg) in Saint Helena?
Answer: You can purchase Tris Base (Ultrapure, 5 kg) from Ubuy, which provides a reliable option for acquiring quality scientific products online. Simply visit the Ubuy website and search for Tris Base, where you'll find various options to choose from, making it easy to find the right product for your laboratory needs.
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Pros
- Good for biochemical applications
- High purity level
- Effective pH buffer
- Reliable performance
- Versatile usage
Cons
- Requires careful handling.
Product Price History
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SHP 493
Order now and get it around Monday, July 06
This item is not restrict in my country.(Please click on above link if this item is not restrict in your country, So our team will review and allow.)
QTY:
Secured transaction
Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
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Features & Benefits
- ULTRA PURE grade for maximum reliability.
- Assay range of 99.9-101% for exceptional purity.
- White crystalline appearance for visual assurance.
- Molecular weight of 121.14 g/mol for precise calculations.
- Convenient storage at room temperature.
- Ideal for scientific and laboratory applications.